2-Methyl-2,4-pentanediol (MPD) boosts as detergent-substitute the performance of ß-barrel hybrid catalyst for phenylacetylene polymerization

• Julia Kinzel,
• Daniel F. Sauer,
• Marco Bocola,
• Marcus Arlt,
• Tayebeh Mirzaei Garakani,
• Andreas Thiel,
• Klaus Beckerle,
• Tino Polen,
• Jun Okuda and
• Ulrich Schwaneberg

Beilstein J. Org. Chem. 2017, 13, 1498–1506, doi:10.3762/bjoc.13.148

• over micelle-forming detergents is demonstrated in the polymerization of phenylacetylene, showing a ten-fold increase in yield and increased molecular weights. Keywords: amphiphilic molecule 2-methyl-2,4-pentanediol; hybrid catalyst; phenylacetylene polymerization; refolding agents; transmembrane

• block copolymer PE–PEG and amphiphilic MPD are used, respectively. Refolding is achieved by dialysis of the protein in a solution containing the particular refolding agents. The structural integrity of FhuA ΔCVFtev was confirmed with CD spectroscopy (Figure 3). When either PE–PEG or MPD is applied, the

• polymer or small amphiphilic molecule. Refolding agents are essential to solubilize transmembrane proteins and keep membrane proteins refolded by shielding hydrophobic residues in aqueous environments. Refolding of the open channel protein FhuA ΔCVFtev was on one hand achieved with polyethylene